Matarazzo was born in Long Island, New York to Camille, a homemaker, and Ray Matarazzo, a data processing manager. She attended Oyster Bay High School. Matarazzo is of fully Irish descent, and was adopted and raised by a strict Catholic Italian American family. In August 2004, Matarazzo openly came out as a lesbian. She has been in a relationship since 2003. In April 2005, she was a speaker at the Gay, Lesbian and Straight Education Network conference in Boston.